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| No. | Valid Name | Author | English Name |
| 1. | Gambusia affinis | (Baird & Girard, 1853) | Mosquitofish |
| 2. | Gambusia alvarezi | Hubbs & Springer, 1957 | Yellowfin gambusia |
| 3. | Gambusia amistadensis | Peden, 1973 | Amistad gambusia |
| 4. | Gambusia atrora | Rosen & Bailey, 1963 | Blackfin gambusia |
| 5. | Gambusia aurata | Miller & Minckley, 1970 | Golden gambusia |
| 6. | Gambusia baracoana | Rivas, 1944 | Baracoa gambusia |
| 7. | Gambusia beebei | Myers, 1935 | Miragoane gambusia |
| 8. | Gambusia bucheri | Rivas, 1944 | Moa gambusia |
| 9. | Gambusia clarkhubbsi | Garrett & Edwards, 2003 | San Felipe gambusia |
| 10. | Gambusia dominicensis | Regan, 1913 | Dominican gambusia |
| 11. | Gambusia echeagarayi | (Álvarez, 1952) | Maya gambusia |
| 12. | Gambusia eurystoma | Miller, 1975 | Widemouth gambusia |
| 13. | Gambusia gaigei | Hubbs, 1929 | Big Bend gambusia |
| 14. | Gambusia geiseri | Hubbs & Hubbs, 1957 | Largespring gambusia |
| 15. | Gambusia georgei | Hubbs & Peden, 1969 | San Marcos gambusia |
| 16. | Gambusia heterochir | Hubbs, 1957 | Clear Creek gambusia |
| 17. | Gambusia hispaniolae | Fink, 1971 | Hispaniolan gambusia |
| 18. | Gambusia holbrooki | Girard, 1859 | Eastern mosquitofish |
| 19. | Gambusia hurtadoi | Hubbs & Springer, 1957 | Crescent gambusia |
| 20. | Gambusia krumholzi | Minckley, 1963 | Spotfin gambusia |
| 21. | Gambusia lemaitrei | Fowler, 1950 | Lemaitre's mosquitofish |
| 22. | Gambusia longispinis | Minckley, 1962 | Cuatrocienegas gambusia |
| 23. | Gambusia luma | Rosen & Bailey, 1963 | Sleek mosquitofish |
| 24. | Gambusia manni | Hubbs, 1927 | Bahamas mosquitofish |
| 25. | Gambusia marshi | Minckley & Craddock, 1962 | Robust gambusia |
| 26. | Gambusia melapleura | (Gosse, 1851) | Striped gambusia |
| 27. | Gambusia monticola | Rivas, 1971 | Yao mosquitofish |
| 28. | Gambusia myersi | Ahl, 1925 | Myers mosquitofish |
| 29. | Gambusia nicaraguensis | Günther, 1866 | Nicaraguan mosquitofish |
| 30. | Gambusia nobilis | (Baird & Girard, 1853) | Pecos gambusia |
| 31. | Gambusia panuco | Hubbs, 1926 | Panuco gambusia |
| 32. | Gambusia pseudopunctata | Rivas, 1969 | Tiburon Peninsula gambusia |
| 33. | Gambusia punctata | Poey, 1854 | Cuban gambusia |
| 34. | Gambusia puncticulata | Poey, 1854 | Caribbean gambusia |
| 35. | Gambusia quadruncus | Langerhans, 2012 | Four hooked mosquitofish |
| 36. | Gambusia regani | Hubbs, 1926 | Forlon gambusia |
| 37. | Gambusia rhizophorae | Rivas, 1969 | Mangrove gambusia |
| 38. | Gambusia senilis | Girard, 1859 | Blotched gambusia |
| 39. | Gambusia sexradiata | Hubbs, 1936 | Teardrop mosquito |
| 40. | Gambusia speciosa | Girard, 1859 | Tex-Mex gambusia |
| 41. | Gambusia vittata | Hubbs, 1926 | Gulf gambusia |
| 42. | Gambusia wrayi | Regan, 1913 | Wray's gambusia |
| 43. | Gambusia xanthosoma | Greenfield, 1983 | Cayman gambusia |
| 44. | Gambusia yucatana | Regan, 1914 | Yucatan gambusia |
| 45. | Gambusia zarskei | Meyer, Schories & Schartl, 2010 | Chihuahua mosquitofish |