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| No. | Valid Name | Author | English Name |
| 1. | Neofundulus acutirostratus | Costa, 1992 | |
| 2. | Neofundulus aureomaculatus | Costa, 2015 | |
| 3. | Neofundulus guaporensis | Costa, 1988 | |
| 4. | Neofundulus ornatipinnis | Myers, 1935 | |
| 5. | Neofundulus paraguayensis | (Eigenmann & Kennedy, 1903) | |
| 6. | Neofundulus parvipinnis | Costa, 1988 | |
| 7. | Neofundulus rubrofasciatus | Costa, 2015 | |
| 8. | Neofundulus splendidus | Nielsen & Brousseau, 2013 |